English
DynEvoLib®

DynEvoLib® is Byongen Therapeutics’ proprietary AAV capsid engineering platform. By integrating four core modules—PEARS, CALIBRATOR, CAMEL, and CAPTURE—it creates a closed-loop, end-to-end system spanning from large-scale library screening to candidate selection. Powered by AI and experimental synergy, DynEvoLib® overcomes R&D bottlenecks like design randomness and signal distortion. This accelerates the transformation of initial sequences into lead variants, significantly enhancing the efficiency and reliability of AAV vector discovery and clinical translation.

  • PEARS
  • CALIBRATOR
  • CAMEL
  • CAPTURE

PEARS is the core module within the DynEvoLib® platform responsible for capsid library construction. This technology integrates random peptide libraries with "surface-informed peptide libraries" generated through rational design and AI assistance, overcoming the coverage limitations inherent in single-type libraries. By analyzing proteomics data, PEARS extracts functional peptide sequences from tens of thousands of cell-surface interacting proteins to construct high-performance AAV capsid-display peptide libraries. Combined with RNA-Seq high-throughput in vivo screening, this module identifies variants with superior transduction efficiency in specific tissues.

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CALIBRATOR is the core module within the DynEvoLib® platform for achieving high-precision screening, specifically designed to correct ranking distortion during AAV library selection. By introducing an in vivo gradient internal control system and leveraging the Standard-curve-based Regression Calibration (SCRC) algorithm, this module enables the quantitative analysis of vector transduction levels. CALIBRATOR systematically filters out experimental noise and preparation bias, ensuring the high-confidence identification of candidate molecules with significant clinical potential.

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CAMEL addresses the throughput limitations inherent in traditional screening methods that rely on single-animal evaluations. Utilizing epitope tag technology and barcode-specific qPCR detection strategies, this module enables the simultaneous, parallel analysis of multiple AAV variants within a single animal across DNA, mRNA, and protein levels. While significantly reducing experimental animal usage, this evaluation framework effectively eliminates interference from individual biological variability, ensuring the high-precision screening of superior vectors with strong functional potential.

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CAPTURE addresses the technical challenge of extracting "sparse signals" during in vivo AAV screening. Utilizing a sequence-specific probe capture strategy, this module enables the high-efficiency, single-tube processing of large-scale tissue samples (gram-level), significantly enhancing the detection sensitivity of low-abundance sequence signals. While ensuring biological fidelity and data stability, CAPTURE provides core technical support for the capsid evolution in large animal models and difficult-to-transduce tissues.

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